Research Assistant, Cell Culture
Columbia University
- Job Type: Support Staff - Non-Union
- Regular/Temporary: Temporary
- End Date if Temporary: 12/31/2025
- Hours Per Week: 35
- Salary Range: $39,800 - $40,400
Position Summary
This six-month grant-funded Research Assistant will help manage our cell culture facility and assist and perform (1) work with animal and cancer models and (2) molecular biology techniques, including bacteria.
Responsibilities
(85%) Assist in overseeing the functioning of the cell/tissue culture (TC) facility in the laboratory by:
- Restocking and maintaining TC inventory weekly.
- Placing orders for TC consumables.
- Cleaning and maintenance of two TC hoods and two C02 incubators as well as scheduling yearly technical repairs and maintenance of this equipment.
- Keeping stock of liquid nitrogen levels.
- Making FBS stocks and preparing 70% ethanol stocks
- Have experience in:
- Cell culture: Sterile technique, passaging and maintenance of cells lines and primary cells, media/FBS preparation, cryopreservation, familiar with microscopy
- Have experience in:
- (10%) Work with animal and cancer models.
- Animal studies: Cancer models, subcutaneous/intravenous/intraperitoneal injections, surgical techniques, anesthesia, IVIS imaging, mouse colony management.
- (5%) Help with general molecular biology techniques including bacteria.
- Molecular biology techniques: restriction digest, PCR, quantitative PCR, cloning, gel electrophoresis, mutagenesis, Gibson assembly, etc.
- Bacteria culture: Sterile technique, maintenance of bacterial strains, LB broth, transformations.
Minimum Qualifications
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High School Diploma required. Minimum 0-3 months of experience in a research environment.
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The ability to work independently, under minimal supervision, following appropriate training.
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Knowledge of basic chemical and/or biological terminology and techniques.
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Ability to perform work in a neat and orderly manner in accordance with safety regulations.
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Computer literacy: proficiency with Microsoft Office.
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Excellent motivation, interpersonal, communication, and organizational skills.
Preferred Qualifications
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Bachelor's degree in Biology or a closely related scientific field with knowledge in general microbiology, cell biology, molecular biology techniques, and animal studies preferred.
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Experience with animal studies.
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.