RESEARCH TECHNICIAN II

School of Medicine

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

The Patel Lab is in the Department of Neurosurgery, Preston Robert Tisch Brain Tumor Center and the Center for Advanced Genomic Technologies and focuses on single cell genomics in brain tumors. Particular focus of the lab includes single cell analyses of patient samples, development of rationally designed cellular therapies, development and study of complex patient specific tumor models to understand minimal residual disease and tumor evolution during therapy, and use of functional genomics to understand tumor dynamics. The lab is seeking a technician to perform a variety of molecular biology based assays to support multiple projects in the lab.

This position will be focused on supporting work in brain organoid models of glioblastoma. Activities related to this will include complex tissue culture and maintenance of organoids, tissue processing, microscopy, single cell RNA-sequencing and library generation, cloning, and other molecular biology techniques.

Primary responsibilities:

• Lab maintenance (ordering, making common reagents/buffers, competent cells, basic tissue culture maintenance)
• Experimental work related to lab activities including tissue culture, cloning, sample preparation for next generation sequencing under supervision from graduate students/postdoctoral fellows
• Oversight over mouse colony and animal activities.

Ideal candidates will have the following knowledge and/or experience:

- Tissue culture including embryonic stem cells

- Mouse survival surgeries and mouse colony management

- Molecular biology techniques such as cloning, western blots, immunofluorescence staining

- Next generation sequencing library preparation

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.